REDUCTION OF GLYOXYLATE BY LACTATE DEHYDROGENASE
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چکیده
منابع مشابه
Catalysis of both oxidation and reduction of glyoxylate by pig heart lactate dehydrogenase isozyme 1.
Pig heart L-lactate dehydrogenase isozyme 1 was purified by preparative polyacrylamide gel electrophoresis. The amino acid composition, specific activity, and pH optima with several substrates were determined. Catalysis of both the oxidation and the reduction of glyoxylate by this enzyme was studied. Reduction of glyoxylate to glycolate requires DPNH, and the rate is optimal at pH 6.9. Oxidatio...
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Incubation of rat hepatocytes with epinephrine inhibited alanine aminotransferase (ALT) (80%) and aspartate aminotransferase (AST) (53%) activities with no effect on lactate dehydrogenase (LDH) activity. Injection of epinephrine caused a progressive increase with time in hepatic LDH activity, being 52% at 24 h. Preinjection with propranolol eliminated the hormone effect and caused further ...
متن کاملReduction of 3-mercaptopyruvate in rat liver is catalyzed by lactate dehydrogenase.
It has been assumed that the in vivo reduction of 3-mercaptopyruvate, an intermediate of cysteine metabolism, to 3-mercaptolactate is catalyzed by lactate dehydrogenase (EC 1.1.1.27) though no definitive evidence has been presented. In order to examine this assumption, reduction of 3-mercaptopyruvate and its inhibition were studied using rat liver homogenate, lactate dehydrogenase purified from...
متن کاملCharacterization of rabbit lactate dehydrogenase-M and lactate dehydrogenase-H cDNAs. Control of lactate dehydrogenase expression in rabbit muscle.
Two cDNA clones were isolated, one corresponding to the mRNA coding for lactate dehydrogenase-M (LDH-M), the other to the mRNA coding for lactate dehydrogenase-H (LDH-H). The cDNA inserts consist of the entire open reading frame for LDH-M and a partial sequence, from amino acid 117 to 332, for LDH-H. Using these two clones as probes we demonstrate that: (a) the abundance of mRNA is muscle-type ...
متن کاملLACTATE DEHYDROGENASE Elevation ofserum lactate dehydrogenasecommences
Heart tissue injury may release cardiac enzymes into the circulation and elevate serum enzyme levels (LaDue, Wroblewski, and Karmen, 1954). Many enzymes become raised, but three enzymesaspartate aminotransferase (EC 2.6.1.1), creatine kinase (EC 2.7.3.2), and lactate dehydrogenase (EC 1.1.1.27)-have proved of particular diagnostic value. In addition, determination of lactate dehydrogenase isoen...
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ژورنال
عنوان ژورنال: THE JOURNAL OF VITAMINOLOGY
سال: 1966
ISSN: 0022-5398
DOI: 10.5925/jnsv1954.12.210